Two New Colorimetric Methods for Early Detection of Vancomycin and Oxacillin Resistance in Staphylococcus aureus

Two new colorimetric methods for early detection of vancomycin and oxacillin resistance in Staphylococcus aureus.

We developed two colorimetric methods for the detection of vancomycin- and oxacillin-resistant Staphylococcus aureus in </=6 h: (i) a nitrate reductase assay and (ii) a resazurin microplate method. MICs agreed with results obtained by CLSI methods for oxacillin. However, detection of vancomycin resistance required a larger inoculum. These methods may be recommended for the detection of vancomyc...

Unacceptable performance and the lack of reproducibility results in the report of colorimetric methods for early detection of vancomycin and oxacillin resistance in Staphylococcus aureus.

In a recent paper by Coban and colleagues (6), two colorimetric methods for early detection of vancomycin and oxacillin resistance in Staphylococcus aureus were reported. The authors state that the results were compared by calculating essential agreement, absolute agreement, and minor, major, and very major errors, as recommended by the FDA (10). However, in the cited version of the FDA documen...

Vancomycin Resistance in Staphylococcus aureus Strains

Prevalence of Vancomycin Resistance in Methicillin-Resistant Staphylococcus Aureus

Background & Aims: Staphylococcus aureus is one of the most common pathogens in nosocomial infections. Vancomycin is the most important therapeutic drug of choice for treatment of infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Therefore, vancomycin-resistant Staphylococcus aureus (VRSA) or vancomycin-intermediate Staphylococcus aureus (VISA) strains are warning...

Comparison of Susceptibility Testing Methods for the Detection of Methicillin/Oxacillin Resistance in Staphylococcus Aureus

Objective: To compare the accuracy of disk diffusion method and E-test for the detection of methicillin resistance and low-level methicillin-resistance in Staphylococcus aureus (S. aureus) and the PBP2a latex agglutination test for confirmation. Materials and Methods: A total of 76 methicillin resistant S. aureus (MRSA) isolates from different clinical specimens were tested by disk diffusion me...